Living-Cell Microarrays
نویسندگان
چکیده
منابع مشابه
Living Cell Microarrays: An Overview of Concepts
Living cell microarrays are a highly efficient cellular screening system. Due to the low number of cells required per spot, cell microarrays enable the use of primary and stem cells and provide resolution close to the single-cell level. Apart from a variety of conventional static designs, microfluidic microarray systems have also been established. An alternative format is a microarray consistin...
متن کاملLaser-guided assembly of heterotypic three-dimensional living cell microarrays.
We have assembled three-dimensional heterotypic networks of living cells in hydrogel without loss of viability using arrays of time-multiplexed, holographic optical traps. The hierarchical control of the cell positions is achieved with, to our knowledge, unprecedented submicron precision, resulting in arrays with an intercell separation <400 nm. In particular, we have assembled networks of Swis...
متن کاملOn-Chip Living-Cell Microarrays for Network Biology
The recently developed field of systems biology creates a new framework for understanding the molecular basis of physiological or pathophysiological states of cells. Screening modalities that can be used on single cells are needed to study cellular systems biology. The recent development of cellular microarrays has provided a method for the complex molecular analysis of living, single cells (Ch...
متن کاملLaser-Guided Assembly of Heterotypic 3D Living Cell Microarrays
We have assembled three-dimensional heterotypic networks of living cells in hydrogel without loss of viability using arrays of time-multiplexed, holographic optical traps. The hierarchical control of the cell positions is achieved with unprecedented submicron precision, resulting in arrays with an inter-cell separation less than 400nm. In particular, we have assembled networks of Swiss-3T3 fibr...
متن کاملSimple benchtop patterning of hydrogel grids for living cell microarrays.
A living cell microarray consists of an orderly arrangement of cells attached to a solid support such as a glass microscope slide. The chief difficulty of obtaining such arrays is the fabrication of substrates patterned with micro-wells, adhesive spots, or other features to guide orderly cell attachment. Here we report a novel method using woven Nylon mesh to micropattern three-dimensional algi...
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ژورنال
عنوان ژورنال: Annual Review of Biomedical Engineering
سال: 2009
ISSN: 1523-9829,1545-4274
DOI: 10.1146/annurev.bioeng.10.061807.160502